Rabu, 11 Februari 2015

The Right Kit For Solubility Screening

By Beryl Dalton


Protein aggregation affects their availability for certain functions. This calls for solubility screening to establish the formulations or conditions that will protect your proteins. The choice of kit will determine the comprehensiveness or intensity of the results you obtain. The protein samples are solubilized during testing depending on expectations.

The kit allows you to use salt, additives and PH to detect quick aggregation capabilities or properties. This kit allows testing based on several conditions in parallel and label free forms. You are not required to use centrifuge during the testing. Simple mixing and spinning produces the desired results. This means that you can obtain desired results in different circumstances.

Screening kits allow you to rescue protein samples that are reversibly aggregated. The kit also performs incredibly well in protecting proteins from aggregating when under stress. This means that they will be available in the form desired. The trends used on such kits are easily found on the dash board. Your results are easily related to what is conventionally known.

Protein aggregation hinders availability of proteins for certain functions and is therefore undesirable. The main causes of aggregation include prolonged storage, vigorous stirring, elevated temperatures and the presence of ligands or protein binding partners. The best way to avoid aggregation is to make the right decision on PH, stabilization additives and salt.

The screening kit provides different buffers with PH ranging from three to ten as well as solubility enhancers. These enhancers include amino acids, polyols, salts, sugars and reducing agents, among others. This will help you determine the conditions under which your protein sample is protected from aggregation or the appropriate conditions for de-aggregation.

Working with this kit allows you to get over ninety formulations using a single label free experiment. You will use the other six as control to get positive and negative assessments. The expected results include soluble proteins going through the filters while aggregated ones do not. The filtration process will help you get these results.

Protein solubilization tests give the conditions under which your proteins will not aggregate. From such tests, it is observed that filtration processes yield proteins when they are in soluble form. They are available in aggregated form if the test does not show any traces of proteins.

Screening results will point at the conditions in which the proteins exist. The results allow you to identify well behaved proteins that will serve your intended purpose. This confirmation is necessary if expected results are to be obtained when such proteins are used in large scale.

You stand to enjoy numerous benefits using this kit than other methods available in the market. It produces clear results that are easy to interpret based on the conditions you are testing. It also is fast which allows you to get results within an hour. This places it at a better position compared to the trial and error methods used in other quotas.

The experiment does not consume large amounts of proteins and is therefore ideal for large scale testing. Reduction in protein use allows it to be matched with assay agents. A detailed report allows more conclusions and inferences to be drawn. There is no restriction on the assay that can be used.




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